[Code of Federal Regulations]
[Title 7, Volume 3]
[Revised as of January 1, 2001]
From the U.S. Government Printing Office via GPO Access
[CITE: 7CFR91.37]
[Page 223-226]
TITLE 7--AGRICULTURE
CHAPTER I--AGRICULTURAL MARKETING SERVICE \1\ (STANDARDS, INSPECTIONS,
MARKETING PRACTICES), DEPARTMENT OF AGRICULTURE (CONTINUED)
PART 91--SERVICES AND GENERAL INFORMATION--Table of Contents
Subpart I--Fees and Charges
Sec. 91.37 Standard hourly fee rate for laboratory testing, analysis, and other services.
(a) The standard hourly fee rate in this section for the individual
laboratory analyses cover the costs of Science and Technology laboratory
services, including issuance of certificates and personnel and overhead
costs other than the commodity inspection fees referred to in 7 CFR
Secs. 52.42 through 52.46, 52.48 through 52.51, 55.510 through 55.530,
55.560 through 55.570, 58.38 through 58.43, 58.45 through 58.46, 70.71
through 70.72, and 70.75 through 70.78. The hourly fee rates in this
part 91 apply to all processed commodity products, except flue-cured and
burley tobacco, and exclude aflatoxin analyses, citrus juices and
certain citrus products. The printed updated schedules of the laboratory
testing fees for processed fruits and vegetables (7 CFR part 93),
poultry and egg products (7 CFR part 94), and meat and meat products (7
CFR part 98) will be available for distribution by the individual
Laboratory Directors of Science and Technology laboratories listed in
Sec. 91.5. The updated schedules of the laboratory testing fees are also
available for electronic access on the world wide web (www) site at:
http://ams.usda.gov/science. The fees for chemical analysis of
cottonseed associated with grading and novel variety seed certification
under the Plant Variety Protection Act are specified in 7 CFR parts 96
and 97, respectively. Except as otherwise provided in this section,
charges will be made for laboratory analysis at the standard hourly rate
of $45.00 for the time required to perform the service. A minimum charge
of one-quarter hour at $11.25 will be made for service pursuant to each
request or certificate issued.
(b) When a laboratory test service is provided for AMS by a
commercial or State government laboratory, the applicant will be
assessed a fee which covers the costs to the Science and Technology
program for the service provided.
[[Page 224]]
(c) When Science and Technology staff provides applied and
developmental research and training activities for microbiological,
physical and chemical analyses on agricultural commodities the applicant
will be charged a fee on a reimbursable cost basis.
General Schedules of Fees for Official Laboratory Test Services
Performed at the AMS Science and Technology Laboratories for Processed
Commodity Products
Table 1--Single Test Laboratory Fees for Proximate Analyses
------------------------------------------------------------------------
Type of analysis List fee
------------------------------------------------------------------------
Ammonia, Ion Selective Electrode............................. $101.25
Ash, Total................................................... 45.00
Chloride, Salt Titration (Dairy)............................. 22.50
Fat, Acid Hydrolysis (Cheese)................................ 45.00
Fat, Acid Hydrolysis (Mojonnier)............................. 45.00
Fat (Dairy Products except Cheese)........................... 22.50
Fat (Dry Basis).............................................. 67.50
Fat, Ether Extraction (Soxhlet).............................. 45.00
Fat (Kohman Analysis)........................................ 45.00
Fat, Microwave--Solvent Extraction........................... 45.00
Moisture, Distillation....................................... 45.00
Moisture, Oven............................................... 22.50
Moisture (Kohman Analysis)................................... 11.25
Protein, Combustion.......................................... 90.00
Protein, Kjeldahl............................................ 90.00
Salt, Back Titration......................................... 33.75
Salt, Potentiometric......................................... 22.50
Salt (Rapid)................................................. 33.75
------------------------------------------------------------------------
Table 2--Single Test Laboratory Fees for Lipid Related Analyses
------------------------------------------------------------------------
Type of analysis List fee
------------------------------------------------------------------------
Acid Degree Value (Dairy).................................... $45.00
Acidity, Titratable.......................................... 22.50
Density (Specific Gravity)................................... 11.25
Dispersibility (Instant Dry Whole Milk)...................... 67.50
Dispersibility (Moates-Dabbah Method)........................ 22.50
Fat Stability,\1\ AOM........................................ 45.00
Fatty Acid Profile (AOAC-GC method).......................... 180.00
Flash Point Test only........................................ 90.00
Free Fatty Acids............................................. 22.50
Meltability (Process Cheese)................................. 22.50
Peanut Oil Analyses (Oil, Moisture, Free Fatty Acids, 45.00
Ammonia, and Foreign Matter)................................
Any One of the Oilseed Oil Analyses.......................... 22.50
Peroxide Value............................................... 33.75
Smoke Point Test only........................................ 90.00
Smoke Point and Flash Point.................................. 157.50
Solids, Total (Oven Drying).................................. 22.50
Soluble Solids, Refractometer................................ 22.50
------------------------------------------------------------------------
\1\ Peroxide value analysis is required as a prerequisite to the fat
stability test at the additional fee.
Table 3--Single Test Laboratory Fees for Food Additives (Direct and
Indirect)
------------------------------------------------------------------------
Type of analysis List fee
------------------------------------------------------------------------
Amitraz Residue, GLC......................................... $112.50
Antibiotic, Qualitative (Dairy).............................. 22.50
Antibiotic, Quantitative \1\N................................ 393.75
Ascorbates (Qualitative--Meats).............................. 22.50
Ascorbic Acid, Titration..................................... 45.00
Ascorbic Acid, Spectrophotometric............................ 45.00
Brix, Direct Percent Sucrose................................. 22.50
Brix, Dilution............................................... 22.50
Butylated Hydroxyanisole (BHA)............................... 67.50
Butylated Hydroxytoluene (BHT)............................... 67.50
Caffeine, Micro Bailey-Andrew................................ 67.50
Caffeine, Spectrophotometric................................. 78.75
Citric Acid, GLC or HPLC..................................... 67.50
Chlorinated Hydrocarbons:
Pesticides and Industrial Chemicals--
Initial Screen........................................... 180.00
Second Column Confirmation of Analyte.................... 45.00
Confirmation on Mass Spectrometer (Per Residue).......... $90.00
Dextrin (Qualitative)........................................ 22.50
Dextrin (Quantitative)....................................... 135.00
Filth, Heavy (Dairy)......................................... 112.50
Filth, Heavy (Eggs).......................................... 180.00
Filth, Light (Eggs).......................................... 112.50
Filth, Light & Heavy (Eggs Extraneous)....................... 270.00
Fines........................................................ 22.50
Flavor (Dairy)............................................... 11.25
Flavor (Products except Dairy)............................... 33.75
Fumigants:
Initial Screen--
Dibromochloropropane (DBCP).............................. 45.00
Ethylene Dibromide....................................... 45.00
Methyl Bromide........................................... 45.00
Confirmation on Mass Spectrometer--
Each individual fumigant residue......................... $90.00
Glucose (Qualitative)........................................ 33.75
Glucose (Quantitative)....................................... 78.75
Glycerol (Quantitative)...................................... 135.00
Gums......................................................... 135.00
Heavy Metal Screen \2\....................................... 326.25
Mercury, Cold Vapor AA....................................... 135.00
Monosodium Dihydrogen Phosphate.............................. 180.00
Monosodium Glutamate......................................... 180.00
Niacin....................................................... 90.00
Ochratoxin A................................................. 67.50
Odor......................................................... 11.25
Organic Acids (in Eggs)...................................... 180.00
Oxygen....................................................... 22.50
Palatability and Odor: Each Sample........................... 22.50
Penicillin................................................... 67.50
Pyrethrin Residue (Dairy).................................... 180.00
Scorched Particles........................................... 22.50
Sodium, Potentiometric....................................... 45.00
Sodium Benzoate, HPLC........................................ 67.50
Sodium Lauryl Sulfate (SLS).................................. 360.00
Sodium Silicoaluminate (Zeolex).............................. 90.00
Solubility Index............................................. 11.25
Starch, Direct Acid Hydrolysis............................... 90.00
Starch (in Dry Milk)......................................... 22.50
Sugar, Polarimetric Methods.................................. 33.75
Sugar Profile, HPLC-- \3\
One type sugar from HPLC profile........................... 135.00
Each additional type sugar................................. 22.50
Sugars, Non-Reducing......................................... 135.00
Sulfur Dioxide, Direct Titration............................. 45.00
Toluene, Residual............................................ 90.00
Vitamin A, Carr-Price (Dairy)................................ 112.50
Vitamin A, HPLC.............................................. 90.00
Vitamin B1 (Thiamin)......................................... 90.00
Vitamin B2 (Riboflavin)...................................... 90.00
Vitamin D, HPLC (Vitamins D2 and D3), Dairy.................. 382.50
[[Page 225]]
Whey Protein Nitrogen........................................ 33.75
Whey Protein Nitrogen, Kjeldahl.............................. 112.50
Xanthydrol Test For Urea..................................... 67.50
This is an optional test to the extraneous materials
isolation test.
------------------------------------------------------------------------
\1\ Antibiotic testing includes tests for chlorotetracycline,
oxytetracycline, and tetracycline.
\2\ Heavy metal screen includes tests for cadmium, lead, and mercury.
\3\ This profile includes the following components: Dextrose, Fructose,
Lactose, Maltose and Sucrose.
Table 4--Single Test Laboratory Fees for Other Chemical and Physical
Component Analyses
------------------------------------------------------------------------
Type of analysis List fee
------------------------------------------------------------------------
Cheese(Fines)................................................ $11.25
Color, Apparent-Visual....................................... 11.25
Complete Kohman Analysis (Dairy)............................. 45.00
Hot Water Insolubles......................................... $67.50
Linolenic Acid............................................... 90.00
Net Weight (Per Can)......................................... 11.25
Non-Volatile Methylene Chloride Extract...................... 112.50
Overrun for Whipped Topping.................................. 33.75
Particle Size (Ether Wash)................................... 22.50
pH........................................................... 11.25
pH--Quinhydrone (Cheese)..................................... 22.50
Potassium Iodide (Table Salt)................................ 67.50
Protein Reducing Substances.................................. 45.00
Quinic Acid (Cranberry Juice)................................ 78.75
Serum Drainage for Whipped Topping........................... 22.50
Sieve or Particle Size....................................... 22.50
Rate of Wetting (Nondairy Creamer)........................... 22.50
Reducing Sugars.............................................. 90.00
Water Activity............................................... 22.50
Water Insoluble Inorganic Residues (WIIR).................... 90.00
------------------------------------------------------------------------
Table 5--Single Test Laboratory Fees for Microbiological Analyses
------------------------------------------------------------------------
Type of analysis List fee
------------------------------------------------------------------------
Aerobic (Standard) Plate Count............................... $22.50
Anaerobic Bacterial Plate Count.............................. 33.75
Bacillus cereus.............................................. 90.00
Bacterial Direct Microscopic Count........................... 45.00
Coliform Plate Count (Dairy Products)........................ 22.50
Coliform Plate Count, Violet Red Bile Agar (Presumptive 33.75
Coliform Plate Count).......................................
Coliforms, Most Probable Number (MPN) \1\:
Step 1..................................................... 33.75
Step 2..................................................... 22.50
Direct Microscopic Clump Count--(Field Submitted Smears, Less 11.25
Than or Equal To 75 Million Count)..........................
Direct Microscopic Clump Count--(Field Submitted Smears, 45.00
Greater Than 75 Million Count)..............................
Direct Microscopic Clump Count--(Lab Prepared Smears)........ 45.00
E. coli, Presumptive MPN (Additional) \2\.................... $45.00
E. coli (MUG \3\)............................................ 33.75
Enterococci Count............................................ 135.00
Howard Mold Count \4\........................................ 56.25
Lactobacillus Count \5\...................................... 56.25
Lactic Acid Tolerant Microbes................................ 22.50
Listeria monocytogenes Confirmation Analysis \6\:
Step 1..................................................... 67.50
Step 2..................................................... 56.25
Step 3 (Confirmation)...................................... 112.50
Parasite Identification...................................... 180.00
Psychrotrophic Bacterial Plate Count......................... 45.00
Salmonella (USDA Culture Method) \7\:
Step 1..................................................... 78.75
Step 2..................................................... 33.75
Step 3 (Confirmation)...................................... 56.25
Salmonella Enumeration (Complete Test)....................... 135.00
Salmonella (Rapid Methods) \8\:
Step 1..................................................... 78.75
Step 2..................................................... 33.75
Step 3 (Confirmation)...................................... 56.25
Salmonella typhi (Meat Products) \9\......................... 45.00
Staphylococcus aureus, Direct Plating........................ 67.50
Staphylococcus aureus, MPN: With Coagulase Positive 78.75
Confirmation................................................
Thermoduric Bacterial Plate Count............................ 33.75
Yeast and Mold Count......................................... 22.50
Yeast and Mold Differential Confirmation..................... 22.50
Yeast and Mold Differential Plate Count...................... 33.75
Yeast or Mold Confirmation................................... 22.50
------------------------------------------------------------------------
\1\ Coliform MPN analysis may be in two steps as follows: Step 1--
presumptive test through lauryl sulfate tryptose broth; Step 2--
confirmatory test through brilliant green lactose bile broth.
\2\ Step 1 of the coliform MPN analysis is a prerequisite for the
performance of the presumptive E. coli test. Prior enrichment in
lauryl sulfate tryptose broth is required for optical recovery of
E.coli from inoculated and incubated EC broth (Escherichia coli
broth). The E. coli test is performed through growth on eosin
methylene blue agar. The fee stated for E. coli analysis is a
supplementary charge to step 1 of coliform test.
\3\ In the presence of the substrate 4-methylumbelliferone--D-
glucuronide (MUG), the enzyme -glucuronidase, which is found
in the majority of E. coli strains, produces a fluorogenic end product
which is visible under ultraviolet (UV) light.
\4\ Howard Mold Count involves counting mold filaments in commodity
products.
\5\ Determination of bacterial plate count of different species of
Lactobacillus.
\6\ Listeria monocytogenes test using the USDA method may be in three
steps as follows: Step 1--isolation by University of Vermont modified
(UVM) broth and Fraser's broth enrichments and selective plating with
Modified Oxford (MOX) agar; Presumptive Step 2--typical colonies
inoculated from Horse Blood into brain heart infusion (BHI) broth and
check for characteristic motility; Confirmatory Step 3--culture from
BHI broth with typical motility is inoculated into the seven
biochemical medias, BHI agar for oxidase and catalase tests, Motility
test medium, and Christie-Atkins-Munch-Peterson (CAMP) test.
Listeria monocytogenes test using the FDA method may be in three steps
as follows: Step 1--isolation by trypticase soy broth with 0.6% yeast
extract (TSB-YE) broth enrichment and selective plating with Modified
McBrides agar and Lithium chloride Phenylethanol Moxalactam (LPM)
agar; Presumptive Step 2--typical colonies inoculated to trypticase
soy agar with yeast extract (TSA-YE) with sheep blood plates to check
for hemolysis followed by inoculations to BHI broth and TSA-YE plates
to check for characteristic motility, gram stain and catalase test;
Confirmatory Step 3--culture from BHI broth with typical motility for
wet mount is inoculated into the required 10 biochemical medias,
Sulfide-Indole-Motility (SIM) medium, and the CAMP test. Serology is
checked using growth from TSA-YE plates.
Both methods for Listeria determination have the equivalent time needed
for each step.
\7\ Salmonella test may be in three steps as follows: Step 1-- growth
through differential agars; Step 2--growth and testing through triple
sugar iron and lysine iron agars; Step 3-- confirmatory test through
biochemicals, and polyvalent serological testing with Poly ``O'' and
Poly ``H'' antiserums. The serological typing of Salmonella is
requested on occasion.
[[Page 226]]
\8\ Salmonella test may be in three steps as follows: Step 1--growth in
enrichment broths and ELISA test or DNA hybridization system assay;
Step 2--growth and testing through triple sugar iron and lysine iron
agars; Step 3--confirmatory test through biochemicals, and polyvalent
serological testing with Poly ``O'' and Poly ``H'' antiserums.
\9\ Salmonella typhi determination in mechanically deboned meat.
Table 6--Laboratory Fees for Aflatoxin Analyses
------------------------------------------------------------------------
Single Pair
Aflatoxin test by commodity analysis analyses \1\
------------------------------------------------------------------------
Peanut Butter (TLC-CB, HPLC, Affinity Column) $45.00 \2\ NA
Corn (TLC-CB, HPLC, Affinity Column)......... 45.00 NA
Roasted Peanuts (TLC-BF)..................... 45.00 NA
Brazil Nuts (TLC-BF)......................... 90.00 NA
Pistachio Nuts (TLC-BF, HPLC)................ 90.00 NA
Shelled Peanuts (TLC, Affinity Column)....... 45.00 $38.00
Shelled Peanuts (HPLC)....................... 45.00 70.00
Tree Nuts (TLC).............................. 45.00 NA
Oilseed Meals (TLC, HPLC, Affinity Column)... $45.00 NA
Edible Seeds (TLC)........................... 45.00 NA
Dried Fruit (TLC)............................ 45.00 NA
Small Grains (TLC)........................... 45.00 NA
In-Shell Peanuts Affinity Column (TLC)....... 45.00 38.00
In-Shell Peanuts (HPLC)...................... 45.00 70.00
Silage; Other Grains (TLC)................... 45.00 NA
Submitted Samples (TLC, HPLC, Affinity 45.00 NA
Column).....................................
Aflatoxin (Dairy, Eggs)...................... 157.50 NA
------------------------------------------------------------------------
\1\ Aflatoxin testing of raw peanuts under Peanut Marketing Agreement
for subsamples 1-AB, 2-AB, 3-AB, and 1-CD for single or pair of
analyses is $19.00 or $38.00, respectively using Thin-Layer
Chromatography (TLC) and Best Foods (BF) extraction or immunoaffinity
column assay with fluorometric quantitation. The BF method has been
modified to incorporate a water slurry extraction procedure. The
Contaminants Branch (CB) method is used on occasion as an alternative
method for peanuts and peanut meal when doubt exists as to the
effectiveness of the Best Foods method in extracting aflatoxin from
the sample or when background interferences exist that might mask TLC
quantitation of aflatoxin. The cost per single or pair of analyses
using High Pressure Liquid Chromatography (HPLC) is $35.00 and $70.00,
respectively. Other aflatoxin for fruits and vegetables are listed at
Science and Technology's current hourly rate of $45.00.
\2\ NA denotes not applicable.
Table 7--Miscellaneous Charges Supplemental to the Science and
Technology's Laboratory Analysis Fees
------------------------------------------------------------------------
Laboratory service description List fee
------------------------------------------------------------------------
Sample Grinding by Vertical Cutter Mixer $22.50
(VCM).
Sample Grinding Canned Boned Poultry....... $11.25 per can.
Sample Grinding by Dickens Hammer Mill..... $11.25.
Sample Grinding (Meats, Meat Products,
Meals, Ready-to-Eat):
Per pouch or raw sample................ $11.25.
Per tray pack.......................... $22.50.
Composting Multiple Subsamples for an Varies--Preparation fee
Individual Test Sample Unit per subsample. based on $45.00 per hour.
------------------------------------------------------------------------
Table 8--Additional Charges Applicable to the Sample Receipt and
Analysis Report
------------------------------------------------------------------------
Service description List charge
------------------------------------------------------------------------
Courier Expense at Other AMS Laboratories: Varies (based on total
Mileage Charge Set at 32.5 cents Per Mile mileage).
Round Trip from Laboratory to Delivery
Site.
Facsimile Charge (Per Analysis Report)..... $3.20 minimum up to first 3
pages, then $1.50 per
page.
Additional Analysis Report or Extra $22.50 per report or
Certificate (\1/2\ hour charge). certificate reissued.
------------------------------------------------------------------------
[65 FR 64311, Oct. 26, 2000]