[Code of Federal Regulations]
[Title 49, Volume 1]
[Revised as of October 1, 2002]
From the U.S. Government Printing Office via GPO Access
[CITE: 49CFR40.71]

[Page 601-602]
 
                        TITLE 49--TRANSPORTATION
 
          SUBTITLE A--OFFICE OF THE SECRETARY OF TRANSPORTATION
 
PART 40--PROCEDURES FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS--Table of Contents
 
                  Subpart E--Urine Specimen Collections
 
Sec. 40.71  How does the collector prepare the specimens?

    (a) All collections under DOT agency drug testing regulations must 
be split specimen collections.
    (b) As the collector, you must take the following steps, in order, 
after the employee brings the urine specimen to you. You must take these 
steps in the presence of the employee.
    (1) Check the box on the CCF (Step 2) indicating that this was a 
split specimen collection.
    (2) You, not the employee, must first pour at least 30 mL of urine 
from the collection container into one specimen bottle, to be used for 
the primary specimen.
    (3) You, not the employee, must then pour at least 15 mL of urine 
from the collection container into the second specimen bottle to be used 
for the split specimen.

[[Page 602]]

    (4) You, not the employee, must place and secure (i.e., tighten or 
snap) the lids/caps on the bottles.
    (5) You, not the employee, must seal the bottles by placing the 
tamper-evident bottle seals over the bottle caps/lids and down the sides 
of the bottles.
    (6) You, not the employee, must then write the date on the tamper-
evident bottle seals.
    (7) You must then ensure that the employee initials the tamper-
evident bottle seals for the purpose of certifying that the bottles 
contain the specimens he or she provided. If the employee fails or 
refuses to do so, you must note this in the ``Remarks'' line of the CCF 
(Step 2) and complete the collection process.
    (8) You must discard any urine left over in the collection container 
after both specimen bottles have been appropriately filled and sealed. 
There is one exception to this requirement: you may use excess urine to 
conduct clinical tests (e.g., protein, glucose) if the collection was 
conducted in conjunction with a physical examination required by a DOT 
agency regulation. Neither you nor anyone else may conduct further 
testing (such as adulteration testing) on this excess urine and the 
employee has no legal right to demand that the excess urine be turned 
over to the employee.

[65 FR 79526, Dec. 19, 2000, as amended at 66 FR 41951, Aug. 9, 2001]