[Code of Federal Regulations]
[Title 21, Volume 6]
[Revised as of January 1, 2003]
From the U.S. Government Printing Office via GPO Access
[CITE: 21CFR529.2503]

[Page 330-331]
 
                        TITLE 21--FOOD AND DRUGS
 
CHAPTER I--FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN 
                          SERVICES (CONTINUED)
 
PART 529--CERTAIN OTHER DOSAGE FORM NEW ANIMAL DRUGS--Table of Contents
 
Sec.  529.2503  Tricaine methanesulfonate.

    (a) Chemical name. Ethyl-m-amino-benzoate methanesulfonate.
    (b) Sponsor. See Nos. 050378 and 051212 in Sec.  510.600(c) of this 
chapter.
    (c) Conditions of use. (1) It is used for the temporary 
immobilization of fish, amphibians, and other aquatic coldblooded 
animals (poikilotherms) as an aid in handling during manual spawning 
(fish stripping), weighing, measuring, marking, surgical operations, 
transport, photography, and research.
    (2) It is used as follows:
    (i) For fish the drug is added to ambient water at a concentration 
of from 15 to 330 milligrams per liter depending upon the degree of 
anesthetization or sedation desired, the species and size of the fish, 
and the temperature and softness of the water. Preliminary tests of 
solutions must be made with small numbers of fish to determine the 
desired rates of sedation or anesthesia and the appropriate exposure 
times for the specific lots of fish under prevailing conditions.
    (ii) For amphibians and other aquatic coldblooded animals, the drug 
is added to ambient water in concentrations of from 1:1000 to 1:20,000 
depending upon species and stage of development.

[[Page 331]]

    (iii) Do not use within 21 days of harvesting fish for food. Use in 
fish intended for food should be restricted to Ictaluridae, Salmonidae, 
Esocidae, and Percidae, and water temperature should exceed 10 [deg]C. 
(50 [deg]F.). In other fish and in cold-blooded animals, the drug should 
be limited to hatchery or laboratory use.

[40 FR 13881, Mar. 27, 1975, as amended at 49 FR 5748, Feb. 15, 1984; 51 
FR 11439, Apr. 3, 1986; 63 FR 7702, Feb. 17, 1998]