[Code of Federal Regulations]
[Title 40, Volume 21]
[Revised as of July 1, 2004]
From the U.S. Government Printing Office via GPO Access
[CITE: 40CFR141.74]

[Page 450-456]
 
                   TITLE 40--PROTECTION OF ENVIRONMENT
 
         CHAPTER I--ENVIRONMENTAL PROTECTION AGENCY (CONTINUED)
 
PART 141_NATIONAL PRIMARY DRINKING WATER REGULATIONS--Table of Contents
 
                  Subpart H_Filtration and Disinfection
 
Sec. 141.74  Analytical and monitoring requirements.

    (a) Analytical requirements. Only the analytical method(s) specified 
in this paragraph, or otherwise approved by EPA, may be used to 
demonstrate compliance with Sec. Sec. 141.71, 141.72 and 141.73. 
Measurements for pH, turbidity, temperature and residual disinfectant 
concentrations must be conducted by a person approved by the State. 
Measurement for total coliforms, fecal coliforms and HPC must be 
conducted by a laboratory certified by the State or EPA to do such 
analysis. Until laboratory certification criteria are developed for the 
analysis of fecal coliforms and HPC, any laboratory certified for total 
coliforms analysis by the State or EPA is deemed certified for fecal 
coliforms and HPC analysis. The following procedures shall be conducted 
in accordance with the publications listed in the following section. 
This incorporation by reference was approved by the Director of the 
Federal Register in accordance with 5 U.S.C. 552(a) and 1 CFR part 51. 
Copies of the methods published in Standard Methods for the Examination 
of Water and Wastewater may be obtained from the American Public Health 
Association et al., 1015 Fifteenth Street, NW., Washington, DC 20005; 
copies of the Minimal Medium ONPG-MUG Method as set forth in the article 
``National Field Evaluation of a Defined Substrate Method for the 
Simultaneous Enumeration of Total Coliforms and Esherichia coli from 
Drinking Water: Comparison with the Standard Multiple Tube Fermentation 
Method'' (Edberg et al.), Applied and Environmental Microbiology, Volume 
54, pp. 1595-1601, June 1988 (as amended under Erratum, Applied and 
Environmental Microbiology, Volume 54, p. 3197, December, 1988), may be 
obtained from the American Water Works Association Research Foundation, 
6666 West Quincy Avenue, Denver, Colorado, 80235; and copies of the 
Indigo Method as set forth in the article ``Determination of Ozone in 
Water by the Indigo Method'' (Bader and Hoigne), may be obtained from 
Ozone Science & Engineering, Pergamon Press Ltd., Fairview Park, 
Elmsford, New York 10523. Copies may be inspected at the U.S. 
Environmental Protection Agency, Room EB15, 401 M St., SW., Washington, 
DC 20460 or at the National Archives and Records Administration (NARA). 
For information on the availability of this material at NARA, call 202-
741-6030, or go to: http://www.archives.gov/federal--register/code--of--
federal--regulations/ibr--locations.html.
    (1) Public water systems must conduct analysis of pH and temperature 
in accordance with one of the methods listed at Sec. 141.23(k)(1). 
Public water systems must conduct analysis of total coliforms, fecal 
coliforms, heterotrophic bacteria, and turbidity in accordance with one 
of the following analytical methods and by using analytical test 
procedures contained in Technical Notes on Drinking Water Methods, EPA-
600/R-94-173, October 1994, which is available at NTIS PB95-104766.

------------------------------------------------------------------------
            Organism                 Methodology         Citation \1\
------------------------------------------------------------------------
Total Coliform \2\.............  Total Coliform      9221 A, B, C
                                  Fermentation
                                  Technique 3 4 5.
                                 Total Coliform      9222 A, B, C
                                  Membrane Filter
                                  Technique \6\.
                                 ONPG-MUG Test \7\.  9223
Fecal Coliforms \2\............  Fecal Coliform      9221 E
                                  Procedure \8\.
                                 Fecal Coliform      9222 D
                                  Filter Procedure.
Heterotrophic bacteria \2\.....  Pour Plate Method.  9215 B
                                 SimPlate \11\.....
Turbidity......................  Nephelometric       2130 B
                                  Method.
                                 Nephelometric       180.1 \9\
                                  Method.
                                 Great Lakes         Method 2 \10\
                                  Instruments.
                                 Hach FilterTrak...  10133 \12\
------------------------------------------------------------------------
 The procedures shall be done in accordance with the documents listed
  below. The incorporation by reference of the following documents
  listed in footnotes 1, 6, 7 and 9-12 was approved by the Director of
  the Federal Register in accordance with 5 U.S.C. 552(a) and 1 CFR part
  51. Copies of the documents may be obtained from the sources listed
  below. Information regarding obtaining these documents can be obtained
  from the Safe Drinking Water Hotline at 800-426-4791. Documents may be
  inspected at EPA's Drinking Water Docket, 1301 Constitution Avenue,
  NW., EPA West, Room B102, Washington DC 20460 (Telephone: 202-566-
  2426); or at the National Archives and Records Administration (NARA).
  For information on the availability of this material at NARA, call 202-
  741-6030, or go to: http://www.archives.gov/federal--register/code--
  of--federal--regulations/ibr--locations.html.
\1\ Except where noted, all methods refer to Standard Methods for the
  Examination of Water and Wastewater, 18th edition (1992), 19th edition
  (1995), or 20th edition (1998), American Public Health Association,
  1015 Fifteenth Street, NW., Washington, DC 20005. The cited methods
  published in any of these three editions may be used.

[[Page 451]]


\2\ The time from sample collection to initiation of analysis may not
  exceed 8 hours. Systems must hold samples below 10 deg. C during
  transit.
\3\ Lactose broth, as commercially available, may be used in lieu of
  lauryl tryptose broth, if the system conducts at least 25 parallel
  tests between this medium and lauryl tryptose broth using the water
  normally tested, and this comparison demonstrates that the false-
  positive rate and false-negative rate for total coliform, using
  lactose broth, is less than 10 percent.
\4\ Media should cover inverted tubes at least one-half to two-thirds
  after the sample is added.
\5\ No requirement exists to run the completed phase on 10 percent of
  all total coliform-positive confirmed tubes.
\6\ MI agar also may be used. Preparation and use of MI agar is set
  forth in the article, ``New medium for the simultaneous detection of
  total coliform and Escherichia coli in water'' by Brenner, K.P., et.
  al., 1993, Appl. Environ. Microbiol. 59:3534-3544. Also available from
  the Office of Water Resource Center (RC-4100T), 1200 Pennsylvania
  Avenue, NW., Washington DC 20460, EPA/600/J-99/225. Verification of
  colonies is not required.
\7\ The ONPG-MUG Test is also known as the Autoanalysis Colilert System.

\8\ A-1 Broth may be held up to three months in a tightly closed screw
  cap tube at 4 deg. C.
\9\ ``Methods for the Determination of Inorganic Substances in
  Environmental Samples'', EPA/600/R-93/100, August 1993. Available at
  NTIS, PB94-121811.
\10\ GLI Method 2, ``Turbidity'', November 2, 1992, Great Lakes
  Instruments, Inc., 8855 North 55th Street, Milwaukee, Wisconsin 53223.

\11\ A description of the SimPlate method, ``IDEXX SimPlate TM HPC Test
  Method for Heterotrophs in Water'', November 2000, can be obtained
  from IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine
  04092, telephone (800) 321-0207.
\12\ A description of the Hach FilterTrak Method 10133, ``Determination
  of Turbidity by Laser Nephelometry'', January 2000, Revision 2.0, can
  be obtained from; Hach Co., P.O. Box 389, Loveland, Colorado 80539-
  0389. Phone: 800-227-4224.

    (2) Public water systems must measure residual disinfectant 
concentrations with one of the analytical methods in the following 
table. Except for the method for ozone residuals, the disinfectant 
residual methods are contained in the 18th, 19th, and 20th editions of 
Standard Methods for the Examination of Water and Wastewater, 1992, 
1995, and 1998; the cited methods published in any of these three 
editions may be used. The ozone method, 4500-O3 B, is 
contained in both the 18th and 19th editions of Standard Methods for the 
Examination of Water and Wastewater, 1992, 1995; either edition may be 
used. If approved by the State, residual disinfectant concentrations for 
free chlorine and combined chlorine also may be measured by using DPD 
colorimetric test kits. Free and total chlorine residuals may be 
measured continuously by adapting a specified chlorine residual method 
for use with a continuous monitoring instrument provided the chemistry, 
accuracy, and precision remain same. Instruments used for continuous 
monitoring must be calibrated with a grab sample measurement at least 
every five days, or with a protocol approved by the State.

------------------------------------------------------------------------
           Residual                Methodology            Methods
------------------------------------------------------------------------
Free Chlorine.................  Amperometric       4500-Cl D
                                 Titration.
                                DPD Ferrous        4500-Cl F
                                 Titrimetric.
                                DPD Colorimetric.  4500-Cl G
                                Syringaldazine     4500-Cl H
                                 (FACTS).
Total Chlorine................  Amperometric       4500-Cl D
                                 Titration.
                                Amperometric       4500-Cl E
                                 Titration (low
                                 level
                                 measurement).
                                DPD Ferrous        4500-Cl F
                                 Titrimetric.
                                DPD Colorimetric.  4500-Cl G
                                Iodometric         4500-Cl I
                                 Electrode.
Chlorine Dioxide..............  Amperometric       4500-ClO2 C
                                 Titration.
                                DPD Method.......  4500-ClO2 D
                                Amperometric       4500-ClO2 E
                                 Titration.
Ozone.........................  Indigo Method....  4500-O3 B
------------------------------------------------------------------------

    (b) Monitoring requirements for systems that do not provide 
filtration. A public water system that uses a surface water source and 
does not provide filtration treatment must begin monitoring, as 
specified in this paragraph (b), beginning December 31, 1990, unless the 
State has determined that filtration is required in writing pursuant to 
Sec. 1412(b)(7)(C)(iii), in which case the State may specify 
alternative monitoring requirements, as appropriate, until filtration is 
in place. A public water system that uses a ground water source under 
the direct influence of surface water and does not provide filtration 
treatment must begin monitoring as specified in this paragraph (b) 
beginning December 31, 1990, or 6 months after the State determines that 
the ground water source is under the direct influence of surface water, 
whichever is later, unless the State has determined that filtration is 
required in writing pursuant to Sec. 1412(b)(7)(C)(iii), in which case 
the State may specify alternative monitoring requirements, as 
appropriate, until filtration is in place.
    (1) Fecal coliform or total coliform density measurements as 
required by Sec. 141.71(a)(1) must be performed on representative 
source water samples immediately prior to the first or only point of 
disinfectant application. The system must sample for fecal or total 
coliforms at the following minimum frequency each week the system serves 
water to the public:

[[Page 452]]



------------------------------------------------------------------------
                                                                Samples/
                 System size (persons served)                   week\1\
------------------------------------------------------------------------
[lE]500......................................................          1
501 to 3,300.................................................          2
3,301 to 10,000..............................................          3
10,001 to 25,000.............................................          4
25,000............................................          5
------------------------------------------------------------------------
\1\ Must be taken on separate days.

    Also, one fecal or total coliform density measurement must be made 
every day the system serves water to the public and the turbidity of the 
source water exceeds 1 NTU (these samples count towards the weekly 
coliform sampling requirement) unless the State determines that the 
system, for logistical reasons outside the system's control, cannot have 
the sample analyzed within 30 hours of collection.
    (2) Turbidity measurements as required by Sec. 141.71(a)(2) must be 
performed on representative grab samples of source water immediately 
prior to the first or only point of disinfectant application every four 
hours (or more frequently) that the system serves water to the public. A 
public water system may substitute continuous turbidity monitoring for 
grab sample monitoring if it validates the continuous measurement for 
accuracy on a regular basis using a protocol approved by the State.
    (3) The total inactivation ratio for each day that the system is in 
operation must be determined based on the CT99.9 values in 
tables 1.1-1.6, 2.1, and 3.1 of this section, as appropriate. The 
parameters necessary to determine the total inactivation ratio must be 
monitored as follows:
    (i) The temperature of the disinfected water must be measured at 
least once per day at each residual disinfectant concentration sampling 
point.
    (ii) If the system uses chlorine, the pH of the disinfected water 
must be measured at least once per day at each chlorine residual 
disinfectant concentration sampling point.
    (iii) The disinfectant contact time(s) (``T'') must be determined 
for each day during peak hourly flow.
    (iv) The residual disinfectant concentration(s) (``C'') of the water 
before or at the first customer must be measured each day during peak 
hourly flow.
    (v) If a system uses a disinfectant other than chlorine, the system 
may demonstrate to the State, through the use of a State-approved 
protocol for on-site disinfection challenge studies or other information 
satisfactory to the State, that CT99.9 values other than 
those specified in tables 2.1 and 3.1 in this section other operational 
parameters are adequate to demonstrate that the system is achieving the 
minimum inactivation rates required by Sec. 141.72(a)(1).

  Table 1.1--CT Values (CT99.9) for 99.9 Percent Inactivation of Giardia Lamblia Cysts by Free Chlorine at 0.5
                                               [deg]C or Lower \1\
----------------------------------------------------------------------------------------------------------------
                                                                                      pH
                      Residual (mg/l)                       ----------------------------------------------------
                                                             [lE]6.0   6.5    7.0    7.5    8.0    8.5   [lE]9.0
----------------------------------------------------------------------------------------------------------------
[lE]0.4....................................................      137    163    195    237    277    329      390
0.6........................................................      141    168    200    239    286    342      407
0.8........................................................      145    172    205    246    295    354      422
1.0........................................................      148    176    210    253    304    365      437
1.2........................................................      152    180    215    259    313    376      451
1.4........................................................      155    184    221    266    321    387      464
1.6........................................................      157    189    226    273    329    397      477
1.8........................................................      162    193    231    279    338    407      489
2.0........................................................      165    197    236    286    346    417      500
2.2........................................................      169    201    242    297    353    426      511
2.4........................................................      172    205    247    298    361    435      522
2.6........................................................      175    209    252    304    368    444      533
2.8........................................................      178    213    257    310    375    452      543
3.0........................................................      181    217    261    316    382    460      552
----------------------------------------------------------------------------------------------------------------
\1\ These CT values achieve greater than a 99.99 percent inactivation of viruses. CT values between the
  indicated pH values may be determined by linear interpolation. CT values between the indicated temperatures of
  different tables may be determined by linear interpolation. If no interpolation is used, use the CT99.9 value
  at the lower temperature and at the higher pH.


  Table 1.2--CT Values (CT 99.9) for 99.9 Percent Inactivation of Giardia Lamblia Cysts by Free Chlorine at 5.0
                                                    [deg]C\1\
----------------------------------------------------------------------------------------------------------------
                                                                                      pH
                    Free residual (mg/l)                    ----------------------------------------------------
                                                             [lE]6.0   6.5    7.0    7.5    8.0    8.5   [lE]9.0
----------------------------------------------------------------------------------------------------------------
[lE]0.4....................................................       97    117    139    166    198    236      279
  0.6......................................................      100    120    143    171    204    244      291
  0.8......................................................      103    122    146    175    210    252      301
  1.0......................................................      105    125    149    179    216    260      312
  1.2......................................................      107    127    152    183    221    267      320
  1.4......................................................      109    130    155    187    227    274      329
  1.6......................................................      111    132    158    192    232    281      337
  1.8......................................................      114    135    162    196    238    287      345
  2.0......................................................      116    138    165    200    243    294      353
  2.2......................................................      118    140    169    204    248    300      361
  2.4......................................................      120    143    172    209    253    306      368
  2.6......................................................      122    146    175    213    258    312      375
  2.8......................................................      124    148    178    217    263    318      382
  3.0......................................................      126    151    182    221    268    324      389
----------------------------------------------------------------------------------------------------------------
\1\ These CT values achieve greater than a 99.99 percent inactivation of viruses. CT values between the
  indicated pH values may be determined by linear interpolation. CT values between the indicated temperatures of
  different tables may be determined by linear interpolation. If no interpolation is used, use the CT99.9 value
  at the lower temperature, and at the higher pH.


[[Page 453]]


 Table 1.3--CT Values (CT 99.9) for 99.9 Percent Inactivation of Giardia Lamblia Cysts by Free Chlorine at 10.0
                                                    [deg]C\1\
----------------------------------------------------------------------------------------------------------------
                                                                                      pH
                    Free residual (mg/l)                    ----------------------------------------------------
                                                             [lE]6.0   6.5    7.0    7.5    8.0    8.5   [lE]9.0
----------------------------------------------------------------------------------------------------------------
[lE]0.4....................................................       73     88    104    125    149    177      209
  0.6......................................................       75     90    107    128    153    183      218
  0.8......................................................       78     92    110    131    158    189      226
  1.0......................................................       79     94    112    134    162    195      234
  1.2......................................................       80     95    114    137    166    200      240
  1.4......................................................       82     98    116    140    170    206      247
  1.6......................................................       83     99    119    144    174    211      253
  1.8......................................................       86    101    122    147    179    215      259
  2.0......................................................       87    104    124    150    182    221      265
  2.2......................................................       89    105    127    153    186    225      271
  2.4......................................................       90    107    129    157    190    230      276
  2.6......................................................       92    110    131    160    194    234      281
  2.8......................................................       93    111    134    163    197    239      287
  3.0......................................................       95    113    137    166    201    243      292
----------------------------------------------------------------------------------------------------------------
\1\ These CT values achieve greater than a 99.99 percent inactivation of viruses. CT values between the
  indicated pH values may be determined by linear interpolation. CT values between the indicated temperatures of
  different tables may be determined by linear interpolation. If no interpolation is used, use the CT99.9 value
  at the lower temperature, and at the higher pH.


 Table 1.4--CT Values (CT 99.9) for 99.9 Percent Inactivation of Giardia Lamblia Cysts by Free Chlorine at 15.0
                                                    [deg]C\1\
----------------------------------------------------------------------------------------------------------------
                                                                                      pH
                    Free residual (mg/l)                    ----------------------------------------------------
                                                             [lE]6.0   6.5    7.0    7.5    8.0    8.5   [lE]9.0
----------------------------------------------------------------------------------------------------------------
[lE]0.4....................................................       49     59     70     83     99    118      140
  0.6......................................................       50     60     72     86    102    122      146
  0.8......................................................       52     61     73     88    105    126      151
  1.0......................................................       53     63     75     90    108    130      156
  1.2......................................................       54     64     76     92    111    134      160
  1.4......................................................       55     65     78     94    114    137      165
  1.6......................................................       56     66     79     96    116    141      169
  1.8......................................................       57     68     81     98    119    144      173
  2.0......................................................       58     69     83    100    122    147      177
  2.2......................................................       59     70     85    102    124    150      181
  2.4......................................................       60     72     86    105    127    153      184
  2.6......................................................       61     73     88    107    129    156      188
  2.8......................................................       62     74     89    109    132    159      191
  3.0......................................................       63     76     91    111    134    162      195
----------------------------------------------------------------------------------------------------------------
\1\ These CT values achieve greater than a 99.99 percent inactivation of viruses. CT values between the
  indicated pH values may be determined by linear interpolation. CT values between the indicated temperatures of
  different tables may be determined by linear interpolation. If no interpolation is used, use the CT99.9 value
  at the lower temperature, and at the higher pH.


   Table 1.5--CT Values (CT99.9) for 99.9 Percent Inactivation of Giardia Lamblia Cysts by Free Chlorine at 20
                                                    [deg]C\1\
----------------------------------------------------------------------------------------------------------------
                                                                                       pH
                                                              --------------------------------------------------
                     Free residual (mg/l)                       [lE]                                       [lE]
                                                                 6.0    6.5    7.0    7.5    8.0    8.5     9.0
----------------------------------------------------------------------------------------------------------------
[lE] 0.4.....................................................      36     44     52     62     74     89     105
0.6..........................................................      38     45     54     64     77     92     109
0.8..........................................................      39     46     55     66     79     95     113
1.0..........................................................      39     47     56     67     81     98     117
1.2..........................................................      40     48     57     69     83    100     120
1.4..........................................................      41     49     58     70     85    103     123
1.6..........................................................      42     50     59     72     87    105     126
1.8..........................................................      43     51     61     74     89    108     129
2.0..........................................................      44     52     62     75     91    110     132
2.2..........................................................      44     53     63     77     93    113     135
2.4..........................................................      45     54     65     78     95    115     138
2.6..........................................................      46     55     66     80     97    117     141
2.8..........................................................      47     56     67     81     99    119     143
3.0..........................................................      47     57     68     83    101    122     146
----------------------------------------------------------------------------------------------------------------
\1\ These CT values achieve greater than a 99.99 percent inactivation of viruses. CT values between the
  indicated pH values may be determined by linear interpolation. CT values between the indicated temperatures of
  different tables may be determined by linear interpolation. If no interpolation is used, use the CT99.9 value
  at the lower temperature, and at the higher pH.


   Table 1.6--CT Values (CT99.9) for 99.9 Percent Inactivation of Giardia Lamblia Cysts by Free Chlorine at 25
                                              [deg]C\1\ and Higher
----------------------------------------------------------------------------------------------------------------
                                                                                       pH
                                                              --------------------------------------------------
                     Free residual (mg/l)                       [lE]                                       [lE]
                                                                 6.0    6.5    7.0    7.5    8.0    8.5     9.0
----------------------------------------------------------------------------------------------------------------
[lE] 0.4.....................................................      24     29     35     42     50     59      70
0.6..........................................................      25     30     36     43     51     61      73
0.8..........................................................      26     31     37     44     53     63      75
1.0..........................................................      26     31     37     45     54     65      78
1.2..........................................................      27     32     38     46     55     67      80
1.4..........................................................      27     33     39     47     57     69      82
1.6..........................................................      28     33     40     48     58     70      84
1.8..........................................................      29     34     41     49     60     72      86
2.0..........................................................      29     35     41     50     61     74      88
2.2..........................................................      30     35     42     51     62     75      90
2.4..........................................................      30     36     43     52     63     77      92
2.6..........................................................      31     37     44     53     65     78      94
2.8..........................................................      31     37     45     54     66     80      96
3.0..........................................................      32     38     46     55     67     81     97
----------------------------------------------------------------------------------------------------------------
\1\ These CT values achieve greater than a 99.99 percent inactivation of viruses. CT values between the
  indicated pH values may be determined by linear interpolation. CT values between the indicated temperatures of
  different tables may be determined by linear interpolation. If no interpolation is used, use the CT99.9 value
  at the lower temperature, and at the higher pH.


  Table 2.1--CT Values (CT99.9) for 99.9 Percent Inactivation of Giardia Lamblia Cysts by Chlorine Dioxide and
                                                    Ozone\1\
----------------------------------------------------------------------------------------------------------------
                                                                              Temperature
                                                     -----------------------------------------------------------
                                                         < 1                 10        15        20      [gE] 25
                                                       [deg]C   5 [deg]C   [deg]C    [deg]C    [deg]C    [deg]C
----------------------------------------------------------------------------------------------------------------
Chlorine dioxide....................................      63        26        23       19        15        11
Ozone...............................................       2.9       1.9       1.4      0.95      0.72      0.48 
----------------------------------------------------------------------------------------------------------------
\1\ These CT values achieve greater than 99.99 percent inactivation of viruses. CT values between the indicated
  temperatures may be determined by linear interpolation. If no interpolation is used, use the CT99.9 value at
  the lower temperature for determining CT99.9 values between indicated temperatures.


[[Page 454]]


 Table 3.1--CT Values (CT 99.9) for 99.9 Percent Inactivation of Giardia
                     Lamblia Cysts By Chloramines\1\
------------------------------------------------------------------------
                               Temperature
-------------------------------------------------------------------------
 < 1 [deg]C    5 [deg]C    10 [deg]C   15 [deg]C   20 [deg]C   25 [deg]C
------------------------------------------------------------------------
3,800            2,200       1,850       1,500       1,100         750
------------------------------------------------------------------------
\1\ These values are for pH values of 6 to 9. These CT values may be
  assumed to achieve greater than 99.99 percent inactivation of viruses
  only if chlorine is added and mixed in the water prior to the addition
  of ammonia. If this condition is not met, the system must demonstrate,
  based on on-site studies or other information, as approved by the
  State, that the system is achieving at least 99.99 percent
  inactivation of viruses. CT values between the indicated temperatures
  may be determined by linear interpolation. If no interpolation is
  used, use the CT99.9 value at the lower temperature for determining
  CT99.9 values between indicated temperatures.

    (4) The total inactivation ratio must be calculated as follows:
    (i) If the system uses only one point of disinfectant application, 
the system may determine the total inactivation ratio based on either of 
the following two methods:
    (A) One inactivation ratio (CTcalc/CT99.9) is determined 
before or at the first customer during peak hourly flow and if the 
CTcalc/CT99.9 [gE] 1.0, the 99.9 percent Giardia lamblia 
inactivation requirement has been achieved; or
    (B) Successive CTcalc/CT99.9 values, representing 
sequential inactivation ratios, are determined between the point of 
disinfectant application and a point before or at the first customer 
during peak hourly flow. Under this alternative, the following method 
must be used to calculate the total inactivation ratio:

[GRAPHIC] [TIFF OMITTED] TC15NO91.133


lamblia inactivation requirement has been achieved.
    (ii) If the system uses more than one point of disinfectant 
application before or at the first customer, the system must determine 
the CT value of each disinfection sequence immediately prior to the next 
point of disinfectant application during peak hourly flow. The CTcalc/
CT99.9 value of each sequence and
[GRAPHIC] [TIFF OMITTED] TC15NO91.134

    must be calculated using the method in paragraph (b)(4)(i)(B) of 
this section to determine if the system is in compliance with Sec. 
142.72(a).
    (iii) Although not required, the total percent inactivation for a 
system with one or more points of residual disinfectant concentration 
monitoring may be calculated by solving the following equation:
[GRAPHIC] [TIFF OMITTED] TC15NO91.135

    (5) The residual disinfectant concentration of the water entering 
the distribution system must be monitored continuously, and the lowest 
value must be recorded each day, except that if there is a failure in 
the continuous monitoring equipment, grab sampling every 4 hours may be 
conducted in lieu of continuous monitoring, but for no more than 5 
working days following the failure of the equipment, and systems serving 
3,300 or fewer persons may take grab samples in lieu of providing 
continuous monitoring on an ongoing

[[Page 455]]

basis at the frequencies prescribed below:

------------------------------------------------------------------------
                                                                Samples/
                  System size by population                      day\1\
------------------------------------------------------------------------
<500.........................................................          1
501 to 1,000.................................................          2
1,001 to 2,500...............................................          3
2,501 to 3,300...............................................          4
------------------------------------------------------------------------
\1\ The day's samples cannot be taken at the same time. The sampling
  intervals are subject to State review and approval.


If at any time the residual disinfectant concentration falls below 0.2 
mg/l in a system using grab sampling in lieu of continuous monitoring, 
the system must take a grab sample every 4 hours until the residual 
concentration is equal to or greater than 0.2 mg/l.
    (6)(i) The residual disinfectant concentration must be measured at 
least at the same points in the distribution system and at the same time 
as total coliforms are sampled, as specified in Sec. 141.21, except 
that the State may allow a public water system which uses both a surface 
water source or a ground water source under direct influence of surface 
water, and a ground water source, to take disinfectant residual samples 
at points other than the total coliform sampling points if the State 
determines that such points are more representative of treated 
(disinfected) water quality within the distribution system. 
Heterotrophic bacteria, measured as heterotrophic plate count (HPC) as 
specified in paragraph (a)(3) of this section, may be measured in lieu 
of residual disinfectant concentration.
    (ii) If the State determines, based on site-specific considerations, 
that a system has no means for having a sample transported and analyzed 
for HPC by a certified laboratory under the requisite time and 
temperature conditions specified by paragraph (a)(3) of this section and 
that the system is providing adequate disinfection in the distribution 
system, the requirements of paragraph (b)(6)(i) of this section do not 
apply to that system.
    (c) Monitoring requirements for systems using filtration treatment. 
A public water system that uses a surface water source or a ground water 
source under the influence of surface water and provides filtration 
treatment must monitor in accordance with this paragraph (c) beginning 
June 29, 1993, or when filtration is installed, whichever is later.
    (1) Turbidity measurements as required by Sec. 141.73 must be 
performed on representative samples of the system's filtered water every 
four hours (or more frequently) that the system serves water to the 
public. A public water system may substitute continuous turbidity 
monitoring for grab sample monitoring if it validates the continuous 
measurement for accuracy on a regular basis using a protocol approved by 
the State. For any systems using slow sand filtration or filtration 
treatment other than conventional treatment, direct filtration, or 
diatomaceous earth filtration, the State may reduce the sampling 
frequency to once per day if it determines that less frequent monitoring 
is sufficient to indicate effective filtration performance. For systems 
serving 500 or fewer persons, the State may reduce the turbidity 
sampling frequency to once per day, regardless of the type of filtration 
treatment used, if the State determines that less frequent monitoring is 
sufficient to indicate effective filtration performance.
    (2) The residual disinfectant concentration of the water entering 
the distribution system must be monitored continuously, and the lowest 
value must be recorded each day, except that if there is a failure in 
the continuous monitoring equipment, grab sampling every 4 hours may be 
conducted in lieu of continuous monitoring, but for no more than 5 
working days following the failure of the equipment, and systems serving 
3,300 or fewer persons may take grab samples in lieu of providing 
continuous monitoring on an ongoing basis at the frequencies each day 
prescribed below:

------------------------------------------------------------------------
                                                                Samples/
                  System size by population                     day \1\
------------------------------------------------------------------------
500....................................          1
501 to 1,000.................................................          2
1,001 to 2,500...............................................          3
2,501 to 3,300...............................................         4
------------------------------------------------------------------------
\1\ The day's samples cannot be taken at the same time. The sampling
  intervals are subject to State review and approval.


If at any time the residual disinfectant concentration falls below 0.2 
mg/l in a system using grab sampling in lieu of continuous monitoring, 
the system must take a grab sample every 4 hours

[[Page 456]]

until the residual disinfectant concentration is equal to or greater 
than 0.2 mg/l.
    (3)(i) The residual disinfectant concentration must be measured at 
least at the same points in the distribution system and at the same time 
as total coliforms are sampled, as specified in Sec. 141.21, except 
that the State may allow a public water system which uses both a surface 
water source or a ground water source under direct influence of surface 
water, and a ground water source to take disinfectant residual samples 
at points other than the total coliform sampling points if the State 
determines that such points are more representative of treated 
(disinfected) water quality within the distribution system. 
Heterotrophic bacteria, measured as heterotrophic plate count (HPC) as 
specified in paragraph (a)(3) of this section, may be measured in lieu 
of residual disinfectant concentration.
    (ii) If the State determines, based on site-specific considerations, 
that a system has no means for having a sample transported and analyzed 
for HPC by a certified laboratory under the requisite time and 
temperature conditions specified by paragraph (a)(3) of this section and 
that the system is providing adequate disinfection in the distribution 
system, the requirements of paragraph (c)(3)(i) of this section do not 
apply to that system.

[54 FR 27527, June 29, 1989, as amended at 59 FR 62470, Dec. 5, 1994; 60 
FR 34086, June 29, 1995; 64 FR 67465, Dec. 1, 1999; 67 FR 65252, Oct. 
23, 2002; 67 FR 65901, Oct. 29, 2002]

    Effective Date Note: At 69 FR 38856, June 29, 2004, Sec. 141.74 was 
amended in paragraph (b)(4)(ii) by removing the citation ``Sec. 
142.72(a)'' and adding in its place ``Sec. 141.72(a)''; in paragraph 
(b)(6)(ii) by removing the citation ``(a)(3)'' and adding in its place 
``(a)(1)''; in paragraph (c)(3)(i) by removing the citation ``(a)(3)'' 
and adding in its place ``(a)(1)''; and in paragraph (c)(3)(ii) by 
removing the citation ``(a)(3)'' and adding in its place ``(a)(1)'', 
effective July 29, 2004.