[Code of Federal Regulations]
[Title 9, Volume 1]
[Revised as of January 1, 2006]
From the U.S. Government Printing Office via GPO Access
[CITE: 9CFR113.30]

[Page 633]
 
                  TITLE 9--ANIMALS AND ANIMAL PRODUCTS
 
  CHAPTER I--ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF 
                               AGRICULTURE
 
PART 113_STANDARD REQUIREMENTS--Table of Contents
 
Sec. 113.30  Detection of Salmonella contamination.

    The test for detection of Salmonella contamination provided in this 
section shall be conducted when such a test is prescribed in an 
applicable Standard Requirement or in the filed Outline of Production 
for the product.
    (a) Samples shall be collected from the bulk suspension before 
bacteriostatic or bactericidal agents have been added. When tissue 
culture products are to be tested, 1 ml of tissue extract used as the 
source of cells or 1 ml of the minced tissue per se shall be tested.
    (b) Five ml of the liquid vaccine suspension shall be used to 
inoculate each 100 ml of liquid broth medium (tryptose and either 
selenite F or tetrathionate). The inoculated media shall be incubated 
18-24 hours at 35-37 [deg]C.
    (c) Transfers shall be made to either MacConkey agar or Salmonella-
Shigella agar, incubated for 18-24 hours and examined.
    (d) If no growth typical of Salmonella is noted, the plates shall be 
incubated an additional 18-24 hours and again examined.
    (e) If suspicious colonies are observed, further subculture on 
suitable media shall be made for positive identification. If Salmonella 
is found, the bulk suspension is unsatisfactory.

[38 FR 29888, Oct. 30, 1973]