[Code of Federal Regulations]
[Title 9, Volume 1]
[Revised as of January 1, 2007]
From the U.S. Government Printing Office via GPO Access
[CITE: 9CFR113.32]

[Page 663]
 
                  TITLE 9--ANIMALS AND ANIMAL PRODUCTS
 
  CHAPTER I--ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF 
                               AGRICULTURE
 
PART 113_STANDARD REQUIREMENTS--Table of Contents
 
Sec. 113.32  Detection of Brucella contamination.

    The test for detection of Brucella contamination provided in this 
section shall be conducted when such a test is prescribed in an 
applicable Standard Requirement or in a filed Outline of Production for 
the product.
    (a) One ml of the minced tissue used as the source of cells or 1 ml 
of the extract of the tissue prior to the addition of antibiotics, 
diluent and stabilizer, shall be inoculated onto each of three tryptose 
agar plates and incubated in a 10 percent CO2 atmosphere at a 
temperature of 35-37 [deg]C for at least 7 days.
    (b) If colonies are identified as Brucella, the biological product 
is unsatisfactory.
    (c) If colonies suspicious of Brucella are observed but cannot be 
identified as a Brucella species, either
    (1) The biological product shall be regarded as unsatisfactory and 
destroyed; or
    (2) Further subculture or other procedures shall be carried out 
until a positive identification can be made.

[38 FR 29888, Oct. 30, 1973]